实验室前期研究结果表明, 在国家规定的药食两用物品名单中, 丁香抗氧化活性最强。 前人发现天然产物的抗氧化功能与抗糖基化活性息息相关。 因此, 目的是在此基础上进一步对丁香精油(clove essential oil, CEO)的抗糖基化活性及其中含量最高的组分-丁香酚(Eugenol)与牛血清白蛋白(bovine serum albumin, BSA)的相互作用进行研究。 在低密度脂蛋白(low density lipoprotein, LDL)的非酶糖基化孵育体系中, 光谱测定结果表明CEO对LDL糖基化早期、 中期和末期产物的生成均具有显著的抑制效果, 且对末期产物的抑制作用最强。 采用气相色谱-质谱联用技术(gas chromatography mass spectrometry, GC-MS)对CEO分析发现, CEO中含量最多的组分是丁香酚。 通过多光谱和分子对接对丁香酚与BSA的相互作用研究发现, 紫外-可见(ultraviolet-visible, UV-Vis)光谱表明丁香酚与BSA之间存在相互作用; 在荧光发射光谱中, 随着丁香酚浓度增加, BSA的荧光强度逐渐增强且发生蓝移, 进一步证明了二者之间存在相互作用。 通过计算不同温度下的结合常数发现, 丁香酚与BSA产生相互作用过程中有热力学过程参与, 热力学参数和位点标记竞争试验表明丁香酚通过氢键和范德华力与BSA在位点Ⅰ结合。 随着丁香酚浓度增加, 丁香酚与BSA混合体系的同步荧光(synchronous fluorescence, SF)、 三维荧光(three-dimensional, 3D)和傅里叶变换红外(Fourier transform infrared, FTIR)光谱的信号强度在发生变化的同时也发生了位移, 表明丁香酚的添加使BSA构象发生了改变。 通过分子对接技术进一步验证了丁香酚与BSA间相互作用的试验结果。 该研究为丁香进一步开发应用提供理论支持。

低密度脂蛋白(LDL)非酶糖基化和氧化修饰均是导致动脉粥样硬化(AS)的关键因素。 前人发现, 丁香可有效抑制牛血清白蛋白(BSA)、 高密度脂蛋白(HDL)糖基化修饰, 丁香乙酸乙酯相(EAEC)和丁香油(CBO)均具有很强的抗氧化活性。 为了确定丁香抑制LDL糖基化及氧化所对应的最有效成分, 在前人研究基础上采用光谱学技术对EAEC和CBO抑制LDL糖基化及氧化功能进行了比较。 首先建立LDL-葡萄糖糖基化孵育体系, 通过测定糖基化早期、 中期和末期产物及三维荧光光谱的变化比较CBO和EAEC抑制LDL糖基化修饰的效果。 其次, 建立LDL-CuSO4氧化孵育体系, 通过测定荧光指标和三维荧光光谱、 紫外可见波长扫描来比较两者抑制LDL氧化修饰效果。 结果发现, EAEC和CBO对LDL糖基化修饰过程中产生的早期产物(Amadori产物)、 中期产物(二羰基化合物)及终末期产物(戊糖素和AGEs)均具有很强的抑制效果, 且EAEC的抑制效果均强于CBO; 三维荧光光谱表征得到相同的结果。 在LDL氧化修饰比较中, CBO对色氨酸(Trp)荧光猝灭、 总荧光产物及脂褐素的生成、 赖氨酸(Lys)修饰的抑制效果均显著强于非油成分的EAEC; 三维荧光光谱表征也得到相同的结果; 紫外、 可见全波长扫描反映CBO比EAEC对共轭二烯(CD)生成及光谱红移的抑制效果更强。 研究结果为后续锁定重点成分分离、 纯化及研发丁香抗LDL糖基化及氧化功能食品提供参考。

An experimental research platform is built on Shenguang II high power laser facility for obtaining the equation of state of liquid deuterium which has ability to control the temperature in a range of 12–300 K with an accuracy of ±0.03 K in 80 min. By optimizing the coating processing and cleaning the target, we solve the problems that the residual reflection is too high and serious frosting takes place on the window of the target at low temperature, then we obtain the experimental image with a good signal-to-noise ratio. By using the impedance matching method and velocity interferometer system for any reflector, experimental Hugoniot data of liquid deuterium are obtained at a pressure of about 60 GPa under the output condition of 3ω, 3 ns, 1200 J on Shenguang II high power laser, which agrees well with the other published data in the same pressure regime and provides a good foundation for the next experimental study of liquid deuterium equation in 100 GPa pressure regime.

The coupling efficiency of short-pulse ignition laser energy to hot-spot internal energy directly affects the feasibility of fast ignition. Experimental characterization of the hot spot has attracted much attention. Among temperature, density and neutron yield of fast ignition experiments, the temperature of the hot spot has few available diagnostic methods. Multispectral X-ray imaging of hot-spot continuum emission is expected to give the time evolution of the electron temperature distribution. This article describes electron temperature determination from multispectral imaging, a dual-channel X-ray Kirkpatrick-Baez (KB) microscope designed for two-spectral imaging, and the experimental results of hot-core multispectral imaging of an imploded cone-shell target at the SG-II laser facility.

The X-ray spectrometer used in high-energy-density plasma experiments generally requires both broad X-ray energy coverage and high temporal, spatial, and spectral resolutions for overcoming the difficulties imposed by the X-ray background, debris, and mechanical shocks. By using an elliptical crystal together with a streak camera, we resolve this issue at the SG-II laser facility. The carefully designed elliptical crystal has a broad spectral coverage with high resolution, strong rejection of the diffuse and/or fluorescent background radiation, and negligible source broadening for extended sources. The spectra that are Bragg reflected (23°<θ<38°) from the crystal are focused onto a streak camera slit 18 mm long and about 80 μm wide, to obtain a time-resolved spectrum. With experimental measurements, we demonstrate that the quartz(1011) elliptical analyzer at the SG-II laser facility has a single-shot spectral range of (4.64–6.45) keV, a typical spectral resolution of E/DE = 560, and an enhanced focusing power in the spectral dimension. For titanium (Ti) data, the lines of interest show a distribution as a function of time and the temporal variations of the He-a and Li-like Ti satellite lines and their spatial profiles show intensity peak red shifts. The spectrometer sensitivity is illustrated with a temporal resolution of better than 25 ps, which satisfies the near-term requirements of high-energy-density physics experiments.

It is important to diagnose electron density of a plasma irradiated by lasers for inertial confinement fusion, in high energy density physics and related fields, especially for measuring high-Z plasma near the interface. Use of soft X-ray laser as a probe is an important method in diagnosis of plasma electron density distribution. However, it is difficult to carry out the research in high-Z laser plasma, because of the problem of excessive plasma spontaneous radiation. In view of the characteristics of soft X-ray laser, several specific experimental techniques have been developed. By using these techniques, which can greatly suppress effects of spontaneous radiation, diagnosis of high-Z plasma with soft X-ray laser probe method becomes possible. As a typical example, an experiment of diagnosing gold plasma is performed and clear images are obtained, indicating that the techniques are effective and feasible.

A joint diagnostic system was established for the diagnosis of laser-driven shock wave experiments. The system has high temporal resolution (time resolution ～12 ps) and high spatial resolution (spatial resolution ～7 μm) and fits for diagnostics of the experiment with small sample size and short time physical process. The joint diagnostic system was applied for shock wave measurement on the Shenguang-II laser facility. The passive shock breakout signal and active diagnostic signal were simultaneously obtained. The temporal measurement reliability of the system was verified using a multi-layered target. The experimental results show that the two measurement results were consistent.

Directly driven ablative Rayleigh–Taylor (R–T) instability of modulated CH targets was studied using the faceon X-ray radiography on the Shen-Guang II device. We obtained temporal evolution images of the R–T instability perturbation. The R–T instability growth factor has been obtained by using the methods of fast Fourier transform and seeking difference of light intensity between the peak and the valley of the targets. Through comparing with the the theoretical simulation, we found that the experimental data had a good agreement with the theoretical simulation results before 1.8 ns, and was lower than the theoretical simulation results after that.

用膜系设计软件设计了λ/4-λ/2的W型的双层减反射薄膜，优化了薄膜的光学常量，并使用溶胶-凝胶技术在玻璃基底上成功镀制了该双层折射率梯度的减反射薄膜.用椭圆偏振光谱仪、紫外-可见-近红外分光光度计、原子力显微镜等分析表征了薄膜的性能.结果表明，镀制了该双层薄膜的玻璃在400 nm～800 nm波段平均透过率增加了近6%，同时薄膜显示出了极佳的机械强度.

The fabrication and measurement of isentropic release targets were described. The Al，Au，Ag、Cu and Zn thin foils were cut to hundred micron by apparatus of laser. They are fabricated to isentropic release targets using in the laser equation of state experiments by the assembly. The parameters of targets were measured by NT1100 profiling system. The isentropic release targets were used in "SG II" experiments and good images were obtained.